Active Recombinant Human HPSE, His-tagged

• Cat.No.: HPSE-156H
• Product Overview: Recombinant Human HPSE(Gln36-Ile543), fused with N-terminal 6-His tag, was expressed in CHO Cells.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Description: Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
• Predicted N Terminal: His & K158
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and E64.
• Bio-activity: Measured by its ability to release heparan sulfate from Recombinant Human Syndecan-4.
• Molecular Mass: Predicted Molecular Mass: 43 kDa & 9 kDaSDS-PAGE: 50-60 kDa & 7-9 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: HPSE heparanase [ Homo sapiens (human) ]
• Official Symbol: HPSE
• Synonyms: HPSE; heparanase; HPA; HPA1; HPR1; HPSE1; HSE1; heparanase-1; heparanase exon10-deletion; heparanase exon 9&10 deletion; EC=3.2.-.-; Heparanase 8 kDa subunit; Heparanase 50 kDa subunit
• Gene ID: 10855
• mRNA Refseq: NM_001098540
• Protein Refseq: NP_001092010
• MIM: 604724
• UniProt ID: Q9Y251
• Chromosome Location: 4q21.3
• Pathway: Glycosaminoglycan degradation; Metabolic pathways; Metabolism of carbohydrates
• Function: beta-glucuronidase activity; heparanase activity; protein dimerization activity