Active Recombinant Mouse HPSE Protein, His-tagged

• Cat.No.: HPSE-7838M
• Product Overview: Recombinant mouse HPSE (28-535) protein with a N-terminal 6/His-tag was expressed in myeloma cells.

Specification

• Species: Mouse
• Source: Myeloma
• Tag: His
• Protein Length: 28-535
• Description: This gene encodes an endoglucuronidase enzyme that plays an important role in tumor invasion and metastasis. The encoded preproprotein undergoes proteolytic processing to generate an active heterodimeric enzyme that cleaves the heparan sulfate proteoglycans associated with the cell surface and extracellular matrix. Mice lacking the encoded protein do not show any prominent pathological alterations under normal conditions but fail to develop albuminuria and renal damage in response to drug-induced diabetes.
• Bio-activity: Measured by its ability to release biotinylated heparan sulfate from Recombinant Human Syndecan‑4. 20 ng rmHPSE digestion will result in >50% of OD reduction compared with the Negative Control
• Molecular Mass: 43 kDa (50 kDa subunit) and 9 kDa (8 kDa subunit)
• N-terminal Sequence Analysis: Glu146 and Lys150
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Applications: ELISA
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles
• Storage: 6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl and E64. See Certificate of Analysis for details
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: Hpse heparanase [ Mus musculus (house mouse) ]
• Official Symbol: HPSE
• Synonyms: HPSE; heparanase; H; Hpa; HSE1; Hpr1; heparanase; endo-glucoronidase; endoglycosidase heparanase
• Gene ID: 15442
• mRNA Refseq: NM_152803
• Protein Refseq: NP_690016
• UniProt ID: Q6YGZ1