Active Recombinant Human Heparan Sulfate (glucosamine) 3-O-Sulfotransferase 1, His-tagged

• Cat.No.: HS3ST1-1970H
• Product Overview: RecombinantHuman HS3ST1 protein was expressed in Chinese HamsterOvary cell line. Arg21-His307, with an N-terminal 6-Histag.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 21-307 a.a.
• Description: Heparan sulfate is a highly sulfated polysaccharide that can befound on cell surface and within extracellular matrix. It is typicallycovalently attached to the protein core of proteoglycans, such as syndecansand glypicans. Heparin, on the other hand, can be considered as a highlysulfated version of heparan sulfate that is detached from the protein coreand is predominantly found in mast cells. Both heparin and heparan sulfatecontain disaccharide repeats of uronic acid and N-acetylglucosamineAnd are modified by the same sulfotransferases. The uronic acidresidues can be sulfated at 2-O position by heparan sulfate 2-O sulfotransferase(HS2ST). The N-acetylglucosamine residues can be sulfated at N, 3-O, and 6-O positionsby N-deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases(HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively.
• Form: Supplied as a 0.2 umfiltered solution in TrisHCl and NaCl.
• N-terminalSequence: His
• MolecularWeight: 35 kDa
• Activity: Measuredby its ability to transfer sulfate from PAPS to heparan sulfate. The specificactivity is > 4 pmoles/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 38-50kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.

Gene Information

• Gene Name: HS3ST1 heparan sulfate(glucosamine) 3-O-sulfotransferase 1 [ Homo sapiens ]
• Official Symbol: HS3ST1
• Synonyms: HS3ST1; heparan sulfate(glucosamine) 3-O-sulfotransferase 1; heparan sulfate glucosamine3-O-sulfotransferase 1; 3OST1; 3-OST-1; 3OST; EC 2.8.2.23; h3OST 1; h3-OST-1;Heparan sulfate 3 O sulfotransferase 1; Heparan sulfate D glucosaminyl 3 Osulfotransferase 1; Heparin glucosamine 3 O sulfotransferase; heparan sulfate3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; heparansulfate D-glucosaminyl 3-O-sulfotransferase 1
• Gene ID: 9957
• mRNA Refseq: NM_005114
• Protein Refseq: NP_005105
• MIM: 603244
• UniProt ID: O14792
• Chromosome Location: 4p16
• Pathway: Glycosaminoglycanbiosynthesis - heparan sulfate
• Function: [heparansulfate]-glucosamine 3-sulfotransferase 1 activity; sulfotransferase activity;transferase activity