Recombinant Human HS3ST1, His-tagged

Description :	Heparan sulfate glucosamine 3-O-sulfotransferase 1, also known as HS3ST1, is sulfotransferase that utilizes 3"-phospho-5"-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. This protein catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.
Source :	E. coli
Species :	Human
Tag :	His
Form :	Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT
Molecular Mass :	36.2 kDa
Protein length :	310aa
AA Sequence :	MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH
Purity :	>90% by SDS - PAGE
Applications :	SDS-PAGE
Storage :	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration :	0.25 mg/ml

Gene Name	HS3ST1 heparan sulfate (glucosamine) 3-O-sulfotransferase 1 [ Homo sapiens ]
Official Symbol	HS3ST1
Synonyms	HS3ST1; heparan sulfate (glucosamine) 3-O-sulfotransferase 1; heparan sulfate glucosamine 3-O-sulfotransferase 1; 3OST1; 3-OST-1; h3-OST-1; heparan sulfate 3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; 3OST;
Gene ID	9957
mRNA Refseq	NM_005114
Protein Refseq	NP_005105
MIM	603244
UniProt ID	O14792
Chromosome Location	4p16
Pathway	Glycosaminoglycan biosynthesis - heparan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - heparan sulfate, conserved biosystem; heparan sulfate biosynthesis, organism-specific biosystem; heparan sulfate biosynthesis, conserved biosystem; heparan sulfate biosynthesis (late stages), organism-specific biosystem; heparan sulfate biosynthesis (late stages), conserved biosystem;
Function	[heparan sulfate]-glucosamine 3-sulfotransferase 1 activity; sulfotransferase activity; transferase activity;

COA

Cat.No. :	HS3ST1-2320H
Product Overview :	Recombinant human HS3ST1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.