Active Recombinant Human FCGRT&B2M protein, His-tagged

• Cat.No.: FCGRT&B2M-1425H
• Product Overview: Recombinant Human FCGRT&B2M protein(Ala24-Ser297(FCGRT)&Ile21-Met119(B2M)), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 24-297;21-119 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Loaded Human FCGRT&B2M Heterodimer Protein, His Tag on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Human FCGRT&B2M Heterodimer Protein, His Tag on AR2G Biosensor, can bind Herceptin with an affinity constant of 0.22 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Biotinylated Human Serum Albumin, His,Avitag on SA Biosensor, can bind Human FCGRT&B2M Heterodimer Protein, His Tag with an affinity constant of 4.09 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: Human FCGRT&B2M Heterodimer Protein, His Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 11.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 10 kDa (B2M) respectively due to glycosylation.
• Proteinlength: Ala24-Ser297(FCGRT)&Ile21-Met119(B2M)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ]
• Official Symbol: FCGRT
• Synonyms: FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; alpha chain; FCRN; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; alpha-chain
• Gene ID: 2217
• mRNA Refseq: NM_001136019
• Protein Refseq: NP_001129491
• MIM: 601437
• UniProt ID: P55899