Active Recombinant Cynomolgus / Rhesus macaque FCGRT&B2M protein, His&StrepII-tagged

• Cat.No.: FCGRT & B2M-1534C
• Product Overview: Recombinant Cynomolgus / Rhesus macaque FCGRT&B2M protein(Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)), fused with His and StrepII tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: His&StrepII
• ProteinLength: Ala24-Ser297(FCGRT)&Ile21-Met119(B2M)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.13 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Biotinylated Cynomolgus Serum Albumin, His,Avitag on SA Biosensor, can bind Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag with an affinity constant of 0.684 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 36 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.