Active Recombinant Mouse FCGRT&B2M protein, Avi-His&Twin-Strep-tagged, Biotinylated

• Cat.No.: FCGRT&B2M-1424M
• Product Overview: Biotinylated Recombinant Mouse FCGRT&B2M protein(Ser22-Ser297(FCGRT)&Ile21-Met119(B2M)), fused with Avi, His and Twin-Strep tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His&Strep
• Protein Length: 22-297;21-119 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Loaded Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag on SA Biosensor, can bind Herceptin with an affinity constant of 3.38 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 15 kDa (B2M) respectively due to glycosylation.
• Proteinlength: Ser22-Ser297(FCGRT)&Ile21-Met119(B2M)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgrt Fc receptor, IgG, alpha chain transporter [ Mus musculus ]
• Official Symbol: FCGRT
• Synonyms: FCGRT; Fc receptor, IgG, alpha chain transporter; IgG receptor FcRn large subunit p51; neonatal Fc receptor; igG Fc fragment receptor transporter alpha chain; FcRn
• Gene ID: 14132
• mRNA Refseq: NM_010189
• Protein Refseq: NP_034319