Active Recombinant Porcine FCGRT&B2M protein, His&StrepII-tagged

• Cat.No.: FCGRT & B2M-19P
• Product Overview: Recombinant Porcine FCGRT&B2M protein(Asp 16 - Ser 289 (FCGRT) & Val 21 - His 118 (B2M)), fused with His and StrepII tag, was expressed in HEK293.

Specification

• Species: Porcine
• Source: HEK293
• Tag: His&Strep II
• Protein Length: Asp16-Ser289(FCGRT)&Val21-His118(B2M)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag captured on CM5 Chip via anti-His antibody can bind Herceptin with an affinity constant of 13.8 nM as determined in SPR assay (Biacore 8K).
• Molecular Mass: Porcine FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 12.8 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with a Strep II tag at the C-terminus. The reducing (R) protein migrates as 32-35 kDa (FCGRT) and 14 kDa (B2M) respectively.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.