Acitve Recombinant Human FCGRT & B2M protein(Met1-Ser297 & Met1-Met119), His&Avi-tagged, Biotinylated

• Cat.No.: FCGRT & B2M-1371H
• Product Overview: Recombinant Human FCGRT (NP_004098.1)(Met1-Ser297) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; Recombinant Human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the Human FCGRT/B2M heterodimer was purified and expressed in HEK293. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Species: Human
• Source: HEK293
• Tag: C-His&Avi
• ProteinLength: Met1-Ser297 & Met1-Met119
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA. 2.Loaded Biotinylated Human FcRn recombinant protein (His & Avi Tag) (on SA Biosensor, can bind IgG1 Fc (IgG1) with an affinity constant of 0.073 μM as determined in a BLI assay (ForteBio Octet Red384). 3.Loaded Biotinylated Human FcRn recombinant protein (His & Avi Tag) on SA Biosensor, can bind IgG4 Fc with an affinity constant of 0.070 μM as determined in a BLI assay (ForteBio Octet Red384).
• Molecular Mass: The recombinant heterodimer of Human FCGRT/B2M comprises 399 (300+99) amino acids and has a calculated molecular mass of 45.3 (33.6+11.7) KDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ]
• Official Symbol: FCGRT; B2M
• Synonyms: FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; alpha chain; FCRN; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; alpha-chain
• Gene ID: 2217
• mRNA Refseq: NM_001136019
• Protein Refseq: NP_001129491
• MIM: 601437
• UniProt ID: P55899