Active Recombinant Human Dual Specificity Phosphatase 3, His-tagged
Cat.No. : | DUSP3-2492H |
Product Overview : | Recombinant human DUSP3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 22.6 kDa. |
Availability | March 14, 2025 |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Protein Length : | 1-185aa |
Description : | DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. |
Form : | Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl. |
Bio-activity : | Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate. |
Molecular Mass : | 22.6 kDa (205aa), confirmed by MALDI-TOF. |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP |
Purity : | >95% by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Bradford) |
Publications : |
Excess PLAC8 promotes an unconventional ERK2-dependent EMT in colon cancer. (2014)
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Gene Name | DUSP3 dual specificity phosphatase 3 [ Homo sapiens ] |
Official Symbol | DUSP3 |
Synonyms | DUSP3; dual specificity phosphatase 3; vaccinia virus phosphatase VH1 related , VHR; dual specificity protein phosphatase 3; vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; dual specificity protein phosphatase VHR; serine/threonine specific protein phosphatase; VHR; |
Gene ID | 1845 |
mRNA Refseq | NM_004090 |
Protein Refseq | NP_004081 |
MIM | 600183 |
UniProt ID | P51452 |
Chromosome Location | 17q21 |
Pathway | Activated TLR4 signalling, organism-specific biosystem; ERK/MAPK targets, organism-specific biosystem; ERKs are inactivated, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MAP kinase activation in TLR cascade, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; |
Function | MAP kinase phosphatase activity; hydrolase activity; phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; |
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◆ Cell & Tissue Lysates | ||
DUSP3-001HCL | Recombinant Human DUSP3 cell lysate | +Inquiry |
Not For Human Consumption!
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