Active Recombinant Human Dual Specificity Phosphatase 3

• Cat.No.: DUSP3-1207H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The mammalian dual-specificity protein-tyrosine phosphatase VHR (for VH1-related) has been identified as a novel regulator of extracellular regulated kinases (ERKs). Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes phosphate groups from tyrosine, serine, and threonine residues. It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to act as a phosphatase for several members of the MAP kinase family including ERK1, ERK2, and JNK. It is a target for the ZAP-70 kinase, and phosphorylation of VHR at tyrosine 138 leads to a downregulation of ERK2 activity. Its welldefined biochemistry has made VHR useful in screening assays for compounds that inhibit phosphatases.
• Form: Liquid. Supplied in 50 mM HEPES pH 7.4, 40 mM NaCl, 1 mM EDTA and 1 mM DTT.
• Purity: ≥90% by SDS-PAGE.
• Activity: 1 nmol of Cdc25A will hydrolyze 180 nmol of pNPP per minute at 37°C at pH 6.5.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: DUSP3 dual specificity phosphatase 3 [ Homo sapiens ]
• Synonyms: DUSP3; dual specificity phosphatase 3; VHR; vaccinia virus phosphatase VH1-related; Dual specificity protein phosphatase 3; EC=3.1.3.48; EC=3.1.3.16; Dual specificity protein phosphatase VHR; serine/threonine specific protein phosphatase; vaccinia virus phosphatase VH1-related
• Gene ID: 1845
• mRNA Refseq: NM_004090
• Protein Refseq: NP_004081
• MIM: 600183
• UniProt ID: P51452
• Chromosome Location: 17q21
• Pathway: MAPK signaling pathway; Signalling by NGF
• Function: hydrolase activity, protein tyrosine; phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity