Active Recombinant Human CYLD Isoform 1 Protein, His-Tagged

• Cat.No.: CYLD-01H
• Product Overview: Recombinant Human CYLD Isoform 1 Protein, His-Tagged was expressed in sf21 cell

Specification

• Description: This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: Sf21 insect cells
• Species: Human
• Tag: His
• Bio-activity: Recombinant Human His6-CYLD Isoform 1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-CYLD Isoform 1 concentration of 50-500 nM.
• Molecular Mass: 108 kDa
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 °C as supplied.
  3 months, -70 °C under sterile conditions after opening.
• Storage Buffer: X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 2 mM DTT
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: CYLD CYLD lysine 63 deubiquitinase [ Homo sapiens (human) ]
• Official Symbol: CYLD
• Synonyms: EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2; FTDALS8
• Gene ID: 1540
• mRNA Refseq: NM_001042355.2
• Protein Refseq: NP_001035814.1
• MIM: 605018
• UniProt ID: Q9NQC7