Active Recombinant Human CLEC4E Protein, Fc-tagged

• Cat.No.: CLEC4E-7777H
• Product Overview: Recombinant Human CLEC4E Protein (Arg41-Leu219) with Human IgG1 (Pro100-Lys330) Fc tag at N-terminus was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.
• Source: CHO
• Species: Human
• Tag: Fc
• Bio-activity: Measured by its binding ability in a functional ELISA. When Trehalose 6, 6'-Dimycolate is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human CLEC4E Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.3 μg/mL.
• Molecular Mass: 47 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Protein length: 41-219 a.a.

Gene Information

• Gene Name: CLEC4E C-type lectin domain family 4 member E [ Homo sapiens (human) ]
• Official Symbol: CLEC4E
• Synonyms: CLEC4E; C-type lectin domain family 4 member E; MINCLE; CLECSF9; C-type lectin domain family 4 member E; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; C-type lectin superfamily member 9; macrophage-inducible C-type lectin
• Gene ID: 26253
• mRNA Refseq: NM_014358
• Protein Refseq: NP_055173
• MIM: 609962
• UniProt ID: Q9ULY5