Recombinant Human CLEC4E Protein (G74-L219), His/Strep tagged

• Cat.No.: CLEC4E-0195H
• Product Overview: Recombinant Human 6His-StrepII-TEV-GG-Mincle(G74-L219 end)-Flag Protein was expressed in E. coli.

Specification

• Description: Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions. Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation. Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue.
• Source: E. coli
• Species: Human
• Tag: His&Strep
• Form: Liquid
• Protein length: G74-L219
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: CLEC4E C-type lectin domain family 4, member E [ Homo sapiens (human) ]
• Official Symbol: CLEC4E
• Synonyms: CLEC4E; C-type lectin domain family 4, member E; C type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 9, CLECSF9; C-type lectin domain family 4 member E; mincle; C-type lectin superfamily member 9; macrophage-inducible C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; MINCLE; CLECSF9
• Gene ID: 26253
• mRNA Refseq: NM_014358
• Protein Refseq: NP_055173
• MIM: 609962
• UniProt ID: Q9ULY5

Reviews

10/24/2022

The CLEC4E protein is an exceptional protein solution that exceeds expectations in terms of quality and suitability for my experimental requirements.

01/01/2021

I am confident that utilizing the CLEC4E protein will empower me to uncover crucial insights into cellular mechanisms with precision.

03/11/2020

In addition to its outstanding quality, the CLEC4E protein comes with exceptional technical support from its manufacturer.

Q&As

Can CLEC4E protein be targeted for the treatment of autoimmune diseases?

While the role of CLEC4E in autoimmune diseases is still being investigated, targeting CLEC4E may hold potential for modulating immune responses in certain autoimmune conditions.

How does CLEC4E protein contribute to immune responses?

CLEC4E protein activates immune cells and initiates inflammatory responses upon recognition of pathogen-associated molecular patterns (PAMPs).

Is CLEC4E protein involved in antiviral immune responses?

Yes, CLEC4E is involved in recognizing viral pathogens and triggering immune responses to combat viral infections.

How does CLEC4E protein interact with other immune receptors?

CLEC4E can collaborate with other immune receptors, such as TLRs (Toll-like receptors), to amplify immune responses upon pathogen detection.

Are there any specific diseases where targeting CLEC4E shows promise?

Targeting CLEC4E holds promise in the development of vaccines and immunotherapies against infectious diseases, including tuberculosis and fungal infections.