Active Recombinant Human C2 protein, His-tagged

• Cat.No.: C2-1032H
• Product Overview: Recombinant Human C2 protein(Ala 21 - Leu 752), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 83.0 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala21-Leu752
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 21-752 a.a.

Gene Information

• Gene Name: C2 complement component 2 [ Homo sapiens ]
• Official Symbol: C2
• Synonyms: C2; complement component 2; complement C2; C3/C5 convertase; complement component C2; CO2; DKFZp779M0311
• Gene ID: 717
• mRNA Refseq: NM_000063
• Protein Refseq: NP_000054
• MIM: 613927
• UniProt ID: P06681