Recombinant Human Complement Component 2, His-tagged

• Cat.No.: C2-598H
• Product Overview: Recombinant Human complement component 2 (C2) was produced inHuman Cells (HEK293)and fused with a C-terminal his tag.

Specification

• Cat. No.: C2-598H
• Description: Complement component C2 is part of the classical complement pathway which plays a major role in innate immunity against infection. C2 is a glycoprotein synthesized in liver hepatocytes and several other cell types in extrahepatic tissues. This pathway is triggered by a multimolecular complex C1, and subsequently the single-chain form of C2 is cleaved into two chains referred to C2a and C2b by activated C1. C2a containing a serine protease domain combines with complement component C4b to form the C3 convertase C4b2a which is responsible for C3 activation, and leads to the stimulation of adaptive immune responses via Lectin pathway. Deficiency of C2 (C2D) is the most common genetic deficiency of the complement system, and two types of C2D have been recognized in the context of specific MHC haplotypes. C2D in human is reported to increase susceptibility to infection, and is associated with certain autoimmune diseases, such as rheumatological disorders.
• Molecular Mass: The single-chain form of recombinant human complement component C2 comprises 743 amino acids and has a calculated molecular mass of 82.5kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhC2 is 90-100 kDa as a result of glycosylation.
• Source: Human Cells (HEK293).
• Predicted N Terminal: Ala 21.
• Endotoxin: <1.0EU per 1μg of the cytokine as determined by the LAL method.
• Formulation: Supplied as a 0.2μm filtered solution of 100mM Glycine, 10mM NaCl, 50 mM Tris, pH7.4.
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Purity: >97%, as determined by SDS-PAGE and SEC-HPLC Analysis.
• Stability: Samples are stable for up to twelve months from date of receipt -70°C.
• Storage: Store at –70°C; Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: C2 complement component 2 [ Homo sapiens ]
• Synonyms: C2; complement component 2; CO2; DKFZp779M0311; C3/C5 convertase; complement component C2; Complement C2; complement component 2; EC 3.4.21.43; OTTHUMP00000029277; OTTHUMP00000062690
• Gene ID: 717
• mRNA Refseq: NM_000063
• Protein Refseq: NP_000054
• MIM: 217000
• UniProt ID: P06681
• Chromosome Location: 6p21.3
• Pathway: Complement and coagulation cascades; Systemic lupus erythematosus; Signaling in Immune system
• Function: peptidase activity; serine-type endopeptidase activity

Reviews

02/02/2023

The C2 protein comes highly recommended for its excellent performance in various research applications.

12/25/2020

It has demonstrated exceptional effectiveness in WB experiments, showcasing its reliability and precision in detecting and quantifying specific targets.

05/08/2019

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

Q&As

Can C2 protein be a target for drug development in autoimmune disorders?

Yes, drugs targeting C2 or the complement system are being explored as potential treatments for autoimmune diseases to regulate the immune response.

Can C2 protein be used as a diagnostic marker for immune system disorders?

Yes, the absence or dysfunction of C2 protein can be indicative of immune system disorders, and its levels can be assessed for diagnostic purposes.

Can C2 protein be used in vaccine development?

Understanding C2's role in immune activation may contribute to vaccine design, potentially enhancing the effectiveness of vaccines by targeting the complement system.

How does C2 protein contribute to autoimmune diseases?

Abnormal activation of the complement system, including C2, is associated with some autoimmune diseases where the immune system mistakenly attacks the body's own cells.

Are there genetic factors influencing C2 protein levels and function?

Yes, genetic variations can affect C2 protein expression and function, leading to variations in immune response among individuals.