Active Recombinant Human ARHGAP1 protein, GST-tagged

• Cat.No.: ARHGAP1-36H
• Product Overview: Recombinant Human ARHGAP1 fused with GST tag was expressed in E. coli.

Specification

• Description: p50RhoGAP (sometimes called Cdc42-GAP or Rho GAP) is a GTPase Activating Protein that catalyzes the hydrolysis of GTP for Rac, Rho, and Cdc42 proteins. It appears to be most active for Cdc42 and least active for Rac.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: It is supplied as a lyophilized powder. When reconstituted to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran.
• Bio-activity: Biological activity of full length p50RhoGAP were verified by the ability of the proteins to enhance GTP hydrolysis for GST-RhoA, GST-Rac1 and GST-Cdc42 proteins. At a molar ratio of 1:0.3 (G-protein : full length p50RhoGAP) hydrolysis of bound GTP was at least 75% completed after 5 min at room temperature. Hydrolysis of bound GTP in the absence of RhoGAP was 0% (GST-RhoA), 10% (GST-Cdc42) or 45% (GST-Rac1) after 5 min at room temperature.
• Molecular Mass: This protein is 78 kDa in size and is comprised of the 50 kDa full length p50RhoGAP protein and a 28 kDa GST tag
• Purity: 90%
• Applications: In vitro GAP assays with various Rho proteins;
  Inhibition of Rho proteins in vivo by microinjection.

Gene Information

• Gene Name: ARHGAP1 Rho GTPase activating protein 1 [ Homo sapiens ]
• Official Symbol: ARHGAP1
• Synonyms: ARHGAP1; Rho GTPase activating protein 1; rho GTPase-activating protein 1; Cdc42GAP; CDC42GAP; p50rhoGAP; RhoGAP; p50-RhoGAP; CDC42 GTPase-activating protein; GTPase-activating protein rhoOGAP; rho-type GTPase-activating protein 1; rho-related small GTPase protein activator; RHOGAP; RHOGAP1
• Gene ID: 392
• mRNA Refseq: NM_004308
• Protein Refseq: NP_004299
• MIM: 602732
• UniProt ID: Q07960
• Chromosome Location: 11p11.2
• Pathway: Regulation of CDC42 activity, organism-specific biosystem; Regulation of RAC1 activity, organism-specific biosystem; Rho GTPase cycle, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by Rho GTPases, organism-specific biosystem
• Function: GTPase activator activity; Rac GTPase activator activity; Rho GTPase activator activity; SH3 domain binding; SH3/SH2 adaptor activity; protein binding