Active Recombinant Full Length Human F2 Protein, C-Flag-tagged

• Cat.No.: F2-490HFL
• Product Overview: Recombinant Full Length Human F2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the prothrombin protein (also known as coagulation factor II). This protein is proteolytically cleaved in multiple steps to form the activated serine protease thrombin. The activated thrombin enzyme plays an important role in thrombosis and hemostasis by converting fibrinogen to fibrin during blood clot formation, by stimulating platelet aggregation, and by activating additional coagulation factors. Thrombin also plays a role in cell proliferation, tissue repair, and angiogenesis as well as maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in this gene lead to various forms of thrombosis and dysprothrombinemia. Rapid increases in cytokine levels following coronavirus infections can dysregulate the coagulation cascade and produce thrombosis, compromised blood supply, and organ failure.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: MS digestion standard
• Molecular Mass: 67.5 kDa
• AA Sequence: MAHVRGLQLPGCLALAALCSLVHSQ HVFLAPQQARSLLQRVRRANTFLEE VRKGNLERECVEETCSYEEA FEALESSTATDVFWAKYTACETART PRDKLAACLEGNCAEGLGTNYRGHV NITRSGIECQLWRSRYPHKP EINSTTHPGADLQENFCRNPDSSTT GPWCYTTDPTVRRQECSIPVCGQDQ VTVAMTPRSEGSSVNLSPPL EQCVPDRGQQYQGRLAVTTHGLPCL AWASAQAKALSKHQDFNSAVQLVEN FCRNPDGDEEGAWCYVAGKP GDFGYCDLNYCEEAVEEETGDGLDE DSDRAIEGRTATSEYQTFFNPRTFG SGEADCGLRPLFEKKSLEDK TERELLESYIDGRIVEGSDAEIGMS PWQVMLFRKSPQELLCGASLISDRW VLTAAHCLLYPPWDKNFTEN DLLVRIGKHSRTRYERNIEKISMLE KIYIHPRYNWRENLDRDIALMKLKK PVAFSDYIHPVCLPDRETAA SLLQAGYKGRVTGWGNLKETWTANV GKGQPSVLQVVNLPIVERPVCKDST RIRITDNMFCAGYKPDEGKR
  GDACEGDSGGPFVMKSPFNNRWYQM GIVSWGEGCDRDGKYGFYTHVFRLK KWIQKVIDQFGETRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton

Gene Information

• Gene Name: F2 coagulation factor II, thrombin [ Homo sapiens (human) ]
• Official Symbol: F2
• Synonyms: PT; THPH1; RPRGL2
• Gene ID: 2147
• mRNA Refseq: NM_000506.5
• Protein Refseq: NP_000497.1
• MIM: 176930
• UniProt ID: P00734

Reviews

01/01/2022

Quality control is evident. This product ensures accuracy in our studies.

01/27/2019

Our lab swears by this protein. Fast shipping is a definite bonus.

12/18/2018

Consistently reliable. The shipping speed is appreciated in our fast-paced lab.

Q&As

Can you discuss the role of the F2 gene in the synthesis of vitamin K-dependent clotting factors, and how disruptions in this process may lead to coagulation disorders?

The F2 gene plays a crucial role in the synthesis of vitamin K-dependent clotting factors, including prothrombin. Disruptions in this process, often due to genetic or nutritional factors, can contribute to coagulation disorders, impacting hemostasis and blood clotting.

How is the F2 gene regulated at the transcriptional level, and what factors influence prothrombin synthesis in hepatocytes?

Transcriptional regulation of the F2 gene involves factors like vitamin K-dependent gamma-carboxylation. Hepatocytes synthesize prothrombin under the influence of vitamin K and other coagulation factors.

The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.

The F2 gene encodes prothrombin, a crucial factor in blood coagulation. Prothrombin is converted to thrombin, a key enzyme in the clotting cascade, facilitating fibrin formation.

What are the implications of genetic variations in the F2 gene, particularly polymorphisms, on prothrombin function and susceptibility to thrombotic or bleeding disorders?

Genetic variations in F2, including polymorphisms, can influence prothrombin function. Certain variants may predispose individuals to thrombotic or bleeding disorders.

How does genetic variation in the F2 gene impact prothrombin levels, and what are the consequences for an individual's risk of thrombotic events?

Genetic variations in the F2 gene can influence prothrombin levels, affecting an individual's propensity for thrombotic events. Certain variants may predispose individuals to a higher or lower risk of abnormal blood clot formation.