Active Native Mouse Thrombin

• Cat.No.: Thrombin-61M
• Product Overview: Human, bovine and mouse thrombin are prepared from purified prothrombin using a modification of the Lundblad procedure as described by Nesheim et al.

Specification

• Species: Mouse
• Tag: Non
• Description: Alpha-thrombin is a highly specific serine protease generated by proteolytic activation of the zymogen prothrombin. During coagulation, thrombin cleaves fibrinogen to form fibrin, leading to the ultimate step in coagulation, the formation of a fibrin clot. Thrombin is also responsible for feedback activation of the procofactors factor V and factor VIII. Thrombin has also been reported to activate factor XIII and platelets, and also functions as a vasoconstrictor protein. The procoagulant activity of thrombin is arrested in two ways: 1) inhibition by either heparin cofactor II or the antithrombin III/heparin complex; or 2) complex formation with thrombomodulin. Formation of the thrombin/thrombomodulin complex results in the inability of thrombin to cleave fibrinogen and activate factors V and VIII, but increases the efficiency of thrombin for activation of the anticoagulant, protein C. Thrombin is a two chain enzyme composed of an NH2-terminal "A" chain (Mr=6,000) and a COOH-terminal "B" chain (Mr=31,000) which remain covalently associated through a single disulfide bond. Human thrombin is 13 amino acids shorter than the bovine thrombin due to a thrombin cleavage site on the human protein that is not present in the bovine protein. Thrombin is also utilized for site specific cleavage of fusion proteins expressed in bacteria. A thrombin sensitive site is incorporated between the recombinant protein of interest and peptides or proteins which facilitate purification and/or expression. The target protein is released from the expressed hybrid by cleavage with thrombin. Thrombin can then be easily removed by affinity chromatography.
• Form: 50% glycerol/water (v/v)
• Bio-activity: approximately 3800 NIH units/mg
• Molecular Mass: 36,702
• Purity: >95% by SDS-PAGENOT tissue/cell culture grade. Not tested for endotoxin.

Gene Information

• Gene Name: F2?coagulation factor II [?Mus musculus?(house mouse) ]
• Official Symbol: F2
• Synonyms: Cf2; FII; Cf-2; thrombin; F2
• Gene ID: 14061
• mRNA Refseq: NM_010168
• Protein Refseq: NP_034298
• UniProt ID: P19221
• Chromosome Location: 2 E1; 2 50.63 cM
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem
• Function: calcium ion binding; catalytic activity