Acitve Recombinant Mouse SHH protein(Met1-Gly198), His-tagged

Source :	HEK293
Species :	Mouse
Tag :	His
Protein length :	Met1-Gly198
Form :	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity :	Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 Mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 5-40 μg/mL.
Molecular Mass :	The recombinant Mouse SHH comprises 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin :	< 1.0 EU per μg of the protein as determined by the LAL method
Purity :	> 90 % as determined by SDS-PAGE
Storage :	Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Name	Shh sonic hedgehog [ Mus musculus ]
Official Symbol	SHH
Synonyms	SHH; sonic hedgehog; sonic hedgehog protein; HHG-1; short digits; hemimelic extra toes; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik;
Gene ID	20423
mRNA Refseq	NM_009170
Protein Refseq	NP_033196