Active Recombinant Human SHH Protein, Pre-aliquoted

• Cat.No.: SHH-04H
• Product Overview: Recombinant Human SHH Protein, Cys24 - Gly197 (Cys24lle-lle), with N-terminal Met, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Cys24-Gly197 (Cys24lle-lle), with N-terminal Met
• Description: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1-0.4 μg/mL.
• Molecular Mass: Predicted Molecular Mass:20 kDa
  SDS-PAGE: 22 kDa, reducing conditions
• N-terminal Sequence Analysis: Met
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll Pre-aliquoted directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: SHH sonic hedgehog signaling molecule [ Homo sapiens (human) ]
• Official Symbol: SHH
• Synonyms: SHH; sonic hedgehog signaling molecule; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5; sonic hedgehog protein; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; sonic hedgehog homolog
• Gene ID: 6469
• mRNA Refseq: NM_000193
• Protein Refseq: NP_000184
• MIM: 600725
• UniProt ID: Q15465