VCAM1
-
Official Full Name
vascular cell adhesion molecule 1 -
Overview
Human Vascular Cell Adhesion Molecule-1 is a 100 - 110 kDa, 715 amino acid type I transmembrane glycoprotein typically characterized by the presence of seven C2-type immunoglobulin domains. Its extracellular region is 674 aa in length, followed by a 22 aa transmembrane segment and a 19 aa cytoplasmic tail. In the extracellular region, there are multiple N-linked glycosylation sites, and each C2 domain is closed by a disulfide bridge. There is considerable interspecies VCAM-1 homology, with mouse and rat VCAM-1 showing approximately 75% aa identity to human VCAM-1. Notably, the short 19 aa cytoplasmic tail is absolutely conserved, mouse to human to rat. Cells expressing mouse VCAM-1 bind both mouse and human leukocytes, and this reflects their high degree of aa identity. A number of variants of VCAM-1 are known to occur, all of which are likely the result of alternate gene splicing. In particular, a human six Ig domain molecule is known, and in rabbits, an eight Ig domain form has been identified. There is also a three-C2 domain, 43 kDa GPI-linked form of VCAM-1. Although it binds known VCAM-1 ligands, its function is unclear. Cells known to express VCAM-1 include neurons, endothelial cells, smooth muscle cells, fibroblasts and macrophages.<br/>Soluble VCAM-1 has been identified in culture supernates, blood, and cerebrospinal fluid. In vitro, basal levels of VCAM-1 shedding by unstimulated NIH3T3 cells appear to partially require metalloproteinase activity, while PMA-induced shedding is dependent upon the proteolytic activity of TACE/ADAM17.<br/>Functionally, VCAM-1 binds to both 4 1 and 4 7 integrins. These integrins are expressed on a variety of cells, with VLA-4 found on all leukocytes with the exception of neutrophils. Because of this, VCAM-1/VCAM-1 ligand interactions are undoubtedly key events in the rate and timing of leukocyte extravasation. Other roles proposed for VCAM-1 include the regulation of osteoclastogenesis via a cell-to-cell contact mechanism and the induction of sickle cell adherence to vascular endothelial cells during hypoxemia.<br/>The Human sVCAM-1 Immunoassay is a 2 hour solid-phase ELISA designed to measure human sVCAM-1 in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sVCAM-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sVCAM-1 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that the Human sVCAM-1 immunoassay kit can be used to determine relative mass values for naturally occurring sVCAM-1. -
Synonyms
VCAM1;vascular cell adhesion molecule 1;INCAM-100;Vascular cell adhesion protein 1 precursor;CD-10^6 antigen;CD10^6;DKFZp779G2333;MGC99561;CD10^6 antigen;OTTHUMP00000012650;OTTHUMP00000012651;L1CAM;V-CAM 1;CD106;Vcam-1
Recombinant Proteins
- Mouse
- Human
- Rhesus macaque
- Dog
- Rat
- Rabbit
- Zebrafish
- Mus musculus
- CHO
- Human Cell
- HEK293
- E.coli
- Mammalian cells
- Mouse
- Insect Cell
- Mammalian Cell
- E.coli expression system
- HEK293T
- Human Cells
- Yeast
- mFc
- Fc
- His&Fc
- His
- GST
- Non
- hIgG4
- T7
- His&T7
- His&S
- Fc&Avi
- His&Fc&Avi
- Myc&DDK
- Flag
- mIgG2a
- His&SUMO
Involved Pathway
VCAM1 involved in several pathways and played different roles in them. We selected most pathways VCAM1 participated on our site, such as NF-kappa B signaling pathway,Cell adhesion molecules (CAMs),TNF signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with VCAM1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| African trypanosomiasis | HBA1,F2RL1,IL10,HPR,SELE,PLCB2,PLCB4,FASLG,IDO2,IL1B |
| Leukocyte transendothelial migration | ACTN4,PIK3CA,VASP,JAM3,NCF2,ROCK1,MAPK13,CLDN3,RHOH,MAPK12 |
| HTLV-I infection | HLA-DPB1,RELB,DVL2,ANAPC4,HLA-DQB1,VDAC1,MAP3K14,WNT5A,FDPS,ANAPC5 |
| TNF signaling pathway | TRAF5,TRAF1,MAPK11,CFLAR,CXCL10,NFKBIA,PIK3CB,TNFRSF1A,BIRC3,MMP9 |
| Malaria | CD40,MYD88,CD40LG,SELE,TLR4,KLRK1,HGF,TGFB2,ITGB2L,GYPC |
| Cell adhesion molecules (CAMs) | CLDN17,CTLA4,PVRL2,CLDN19,NLGN4X,ITGAM,ITGB1B.2,NRCAMA,CLDN6,NADL1.2 |
| NF-kappa B signaling pathway | RELA,LAT,PLCG2,GM1987,PRKCQ,TNFRSF1A,LCK,TRAF2,LYN,TRADD |
Protein Function
VCAM1 has several biochemical functions, for example, cell adhesion molecule binding,integrin binding,primary amine oxidase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by VCAM1 itself. We selected most functions VCAM1 had, and list some proteins which have the same functions with VCAM1. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| integrin binding | LAMB2,TLN1,ADAM10,TNN,ITGA3,CYR61L1,ACTN3,CD226,PPAP2B,ADAM9 |
| primary amine oxidase activity | ABP1,MAOB,MAOA,AOC2,AOC1,AOC3 |
| cell adhesion molecule binding | SDCBP,ITGA4,CD200,GLYCAM1,NRXN2,AMICA1,DSP,PKP3,NLGN2,CRTAM |
Interacting Protein
VCAM1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with VCAM1 here. Most of them are supplied by our site. Hope this information will be useful for your research of VCAM1.
ITGB1;ZYX;TLN1;PSMD11
Resources
Research Area
Endothelial Cell MarkersImmunoglobulin Superfamily CAMs
Endothelial Cell Differentiation Markers
Hematopoietic Stem Cell Markers
Mesenchymal Stem Cell Markers
Adipose-derived Stem Cell Markers
CD Antigen (T Cell Migration/Adhesion)
Mesodermal Lineage Markers
Related Services
Related Products
References
- Almeda, D; Wang, BR; et al. Minimizing antibody surface density on liposomes while sustaining cytokine-activated EC targeting. BIOMATERIALS 41:37-44(2015).
- Marschall, AL; Single, FN; et al. Functional knock down of VCAM1 in mice mediated by endoplasmatic reticulum retained intrabodies. MABS 6:1394-1401(2014).
