Active Recombinant Mouse Vcam1, His-tagged

• Cat.No.: Vcam1-4013M
• Product Overview: A DNA sequence encoding the extracellular domain ( Met 1 - Glu 698 ) of mouse VCAM1 ( NP_035823.3 ) was fused with the a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: Mouse
• Tag: His
• Protein Length: 1-698 a.a.
• Description: Mouse vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two isoforms of mouse VCAM1 have been identified, referred to as single-pass type I transmembrane (short) isoform 1 containing seven C2-type I g-like domains and GPI-anchored isoform 2. Mouse VCAM1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells / well are added to mouse VCAM1 coated plates ( 10 μg/mL with 100 μL/well ) , approximately 70% - 80% cells will adhere after 1 hour incubation at RT
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70μ
• Predicted N terminal: Phe 25
• Molecular Mass: The secreted recombinant mouse VCAM1 consists of 685 amino acids and has a predicted molecular mass of 75.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1 is approximately 90-100 kDa due to glycosylation.
• Storage: Store it under sterile conditions at -70μ. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Vcam1 vascular cell adhesion molecule 1 [ Mus musculus ]
• Official Symbol: Vcam1
• Synonyms: Vcam1; vascular cell adhesion molecule 1; CD106; Vcam-1
• Gene ID: 22329
• mRNA Refseq: NM_011693
• Protein Refseq: NP_035823
• UniProt ID: P29533
• Chromosome Location: 3 G1; 3 50.8 cM
• Pathway: Cell adhesion molecules (CAMs); Leukocyte transendothelial migration
• Function: protein binding