Active Recombinant Human VCAM1 Protein

• Cat.No.: VCAM1-01H
• Product Overview: Recombinant Human VCAM1 (Arg322Gly) Protein (Phe25-Glu698) without tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Protein Length: 25-698 a.a.
• Description: This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human sVCAM-1 coated plates (10 μg/mL with 100 μL/well), >85% will adhere after 1 hour incubation at room temperature.
• Molecular Mass: Predicted Molecular Mass: 74.1 kDa
  SDS-PAGE: 93-95 kDa, reducing conditions
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
• Reconstitution: Reconstitute at 1 mg/mL in sterile, deionized water.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: VCAM1 vascular cell adhesion molecule 1 [ Homo sapiens (human) ]
• Official Symbol: VCAM1
• Synonyms: VCAM1; vascular cell adhesion molecule 1; CD106; INCAM-100; vascular cell adhesion protein 1; CD106 antigen
• Gene ID: 7412
• mRNA Refseq: NM_001078
• Protein Refseq: NP_001069
• MIM: 192225
• UniProt ID: P19320