Active Recombinant Mouse Vcam1, His & hFc-tagged

• Cat.No.: Vcam1-1784M
• Product Overview: Recombinant Mouse Vcam1(Met 1-Glu 698)(NP_035823.3) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met 1-Glu 698
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells.
  When cells are added to VCAM1-coated plates (10 μg/ml, 100 μg/well), approximately >70% cells will adhere specifically.
• Molecular Mass: The recombinant mouse VCAM1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 922 amino acids and has a predicted molecular mass of 102 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1/Fc monomer is approximately 110-120 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Vcam1 vascular cell adhesion molecule 1 [ Mus musculus ]
• Official Symbol: Vcam1
• Synonyms: VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; CD106; Vcam-1
• Gene ID: 22329
• mRNA Refseq: NM_011693
• Protein Refseq: NP_035823