Recombinant Xenopus ube2d2, His-tagged

• Cat.No.: ube2d2-236X
• Product Overview: Recombinant full length Xenopus UBE2D2 (UBC4) was expressed in E. coli cells using an N-terminal His tag.

Specification

• Description: UBE2D2(UBC4) Protein or ubiquitin-conjugating enzyme E2D 2 is a member of the E2 ubiquitin-conjugating enzyme family which functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 specifically ubiquitinates E6AP and in vivo inhibition of UBE2D2 can lead to inhibition of E6-stimulated p53 degradation. UBE2D2 could conjugate ubiquitin to target proteins in an E6AP-dependent manner.
• Source: E. coli
• Species: Xenopus laevis
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~17 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: ube2d2?ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) [?Xenopus laevis?(African clawed frog) ]
• Official Symbol: ube2d2
• Synonyms: ube2d2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); EC 6.3.2.-
• Gene ID: 446340
• mRNA Refseq: NM_001093036
• Protein Refseq: ?NP_001086505
• Pathway: Protein processing in endoplasmic reticulum; Ubiquitin mediated proteolysis

Reviews

07/12/2022

Trusted for research integrity, supports research consistency.

10/24/2020

Outstanding accuracy, highly recommended for insights.

12/30/2017

Efficient service, accelerates our research timelines.

Q&As

How does UBE2D2 interact with other components of the ubiquitin-proteasome system?

UBE2D2 works in tandem with other ubiquitin-proteasome system components, orchestrating protein degradation.

How does UBE2D2 contribute to cellular protein degradation?

It facilitates the attachment of ubiquitin to target proteins, directing them to the proteasome for breakdown.

What role does UBE2D2 play in cell cycle regulation?

UBE2D2 plays a role in controlling the cell cycle by regulating the degradation of key cycle-related proteins.

What potential therapeutic applications arise from targeting UBE2D2 in diseases related to protein misfolding?

Targeting UBE2D2 could offer new treatments for conditions involving protein misfolding and aggregation.

What is the impact of altered UBE2D2 activity on disease development?

Altered UBE2D2 activity is linked with several diseases, including cancer and neurodegenerative disorders.

How do genetic variations in UBE2D2 affect cellular homeostasis?

Genetic mutations in UBE2D2 can disrupt protein turnover, affecting various cellular functions and stability.

What is the primary role of UBE2D2 in protein ubiquitination?

UBE2D2, a ubiquitin-conjugating enzyme, is vital for tagging proteins with ubiquitin, marking them for degradation.