Recombinant Human UBE2D2, His-tagged

• Cat.No.: UBE2D2-37H
• Product Overview: Recombinant Human UBE2D2 produced inE. coliwas fused to a his tag.

Specification

• Cat. No.: UBE2D2-37H
• Description: UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53.
• Source: E. coli.
• Purity: 95% (SDS-PAGE).
• Application: Useful forin vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions.
• Storage: -80°C.

Gene Information

• Gene Name: UBE2D2 ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) [ Homo sapiens ]
• Synonyms: UBE2D2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); UBC4; PUBC1; UBC4/5; UBCH5B; E2(17)KB2; ubiquitin-conjugating enzyme E2 D2; ubiquitin-protein ligase D2; ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2(17)KB 2; ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); EC 6.3.2.19; ubiquitin carrier protein
• Gene ID: 7322
• mRNA Refseq: NM_003339
• Protein Refseq: NP_003330
• MIM: 602962
• UniProt ID: P62837
• Chromosome Location: 5q31.2
• Pathway: Ubiquitin mediated proteolysis
• Function: ATP binding; ligase activity; nucleotide binding; protein binding; ubiquitin-protein ligase activity