Recombinant Rat Txnrd1 Protein

• Cat.No.: Txnrd1-001R
• Product Overview: Active recombinant rat TrxR1 protein (3-499) without tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms.
• Source: E. coli
• Species: Rat
• Molecular Mass: 54.5 kDa
• Protein length: 3-499
• Purity: ≥90% as determined by SDS-PAGE
• Unit Definition: One unit is defined as the NADPH-dependent production of 2 mmol of 2-nitro-5-thiobenzoate per minute at 22 centigrade in 50 mM potassium phosphate, pH 7.0, with 50 mM potassium chloride, 1 mM EDTA, and 0.2 mg/mL BSA. For inhibition, the selective TrxR1 inhibitor sodium aurothiomalate (ATM) was used at a concentration of 20 mM.
• Applications: WB, Enzyme activity assay
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM Tris-HCl, pH 7.4, with 2 mM EDTA and 10% glycerol

Gene Information

• Gene Name: Txnrd1 thioredoxin reductase 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Txnrd1
• Synonyms: Txnrd1; thioredoxin reductase 1; Tr; thioredoxin reductase 1, cytoplasmic; NADPH-dependent thioredoxin reductase; selenoprotein oxidoreductase; thioredoxin reductase TR1; EC 1.8.1.9
• Gene ID: 58819
• mRNA Refseq: NM_031614
• Protein Refseq: NP_113802
• UniProt ID: O89049