Recombinant Rat Txnrd1 protein, His & T7-tagged

• Cat.No.: Txnrd1-685R
• Product Overview: Recombinant Rat Txnrd1 aa. (Phe208~Glu433 (Accession # O89049)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the family of pyridine nucleotide-disulfide oxidoreductases. This protein is a flavoenzyme, which uses NADPH for reduction of thioredoxins as well as other protein and nonprotein substrates, and plays a role in protection against oxidative stress. It contains a selenocysteine (Sec) residue, which is essential for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal.
• Source: E. coli
• Species: Rat
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.0kDa
• Protein length: Phe208~Glu433
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Txnrd1 thioredoxin reductase 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Txnrd1
• Synonyms: Txnrd1; thioredoxin reductase 1; Tr; thioredoxin reductase 1, cytoplasmic; NADPH-dependent thioredoxin reductase; selenoprotein oxidoreductase; thioredoxin reductase TR1
• Gene ID: 58819
• mRNA Refseq: NM_031614.2
• Protein Refseq: NP_113802.2
• UniProt ID: O89049