Recombinant Rat Sema4d protein(Met1-Met712), His-tagged

• Cat.No.: Sema4d-8772R
• Product Overview: Recombinant Rat SEMA4D (NP_001164034.1)(Met1-Met712) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Met712
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant rat SEMA4D comprises 700 amino acids and predicts a molecular mass of 77.8 kDa. The apparent molecular mass of the recombinant protein is approximately 96 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sema4d sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D [ Rattus norvegicus ]
• Official Symbol: Sema4d
• Synonyms: SEMA4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Gene ID: 306790
• mRNA Refseq: NM_001170563
• Protein Refseq: NP_001164034