Recombinant Human SEMA4D protein, His-tagged

• Cat.No.: SEMA4D-6946H
• Product Overview: Recombinant Human SEMA4D aa. (Phe502~Phe636) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Phe502~Phe636
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 8.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SEMA4D semaphorin 4D [ Homo sapiens (human) ]
• Official Symbol: SEMA4D
• Synonyms: SEMA4D; semaphorin 4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G; semaphorin-4D; A8; BB18; GR3; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
• Gene ID: 10507
• mRNA Refseq: NM_001142287.1
• Protein Refseq: NP_001135759.1
• UniProt ID: Q92854