Recombinant Human SEMA4D protein, His-Avi-tagged

• Cat.No.: SEMA4D-330H
• Product Overview: Recombinant Human SEMA4D protein(Q92854-1)(Met22-Arg734), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His-Avi
• Protein length: Met22-Arg734
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: SEMA4D sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D [ Homo sapiens ]
• Official Symbol: SEMA4D
• Synonyms: SEMA4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; C9orf164, chromosome 9 open reading frame 164 , SEMAJ; semaphorin-4D; CD100; coll 4; FLJ39737; M sema G; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; SEMAJ; coll-4; C9orf164; M-sema-G; FLJ33485; FLJ34282; FLJ46484; MGC169138; MGC169141
• Gene ID: 10507
• mRNA Refseq: NM_001142287
• Protein Refseq: NP_001135759
• MIM: 601866
• UniProt ID: Q92854