Recombinant Rat SCGB1A1 Protein

• Cat.No.: SCGB1A1-5252R
• Product Overview: Recombinant Rat SCGB1A1 full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Rat
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Scgb1a1 secretoglobin, family 1A, member 1 (uteroglobin) [ Rattus norvegicus ]
• Official Symbol: SCGB1A1
• Gene ID: 25575
• mRNA Refseq: NM_013051.1
• Protein Refseq: NP_037183.1
• UniProt ID: P17559

Citation

Lung Secretoglobin Scgb1a1 Influences Alveolar Macrophage-Mediated Inflammation and Immunity

Journal: Frontiers in Immunology    PubMed ID: 33117399    Data: 2020/10/1

Authors: Min Xu, Wei Yang, Deepak Kumar Nayak

Article Snippet:The effect of SCGB1a1 protein on AM responsiveness to inflammatory stimuli were studied in vitro .The effect of SCGB1a1 protein on AM responsiveness to inflammatory stimuli were studied in vitro .. Flow sorted AMs were plated ( ) in 12-well plates at 1 × 10 5 cells/well in triplicates and were incubated with Toll-like receptor (TLR) agonists (Invivogen) 2- heat-killed Listeria monocytogenes (HKLM), TLR4-Lipopolysaccharide from Escherichia coli K12 (LPS) and TLR5- Salmonella typhimurium Flagellin (FLA) in presence or absence of recombinant SCGB1a1 protein at 5 μg/mL (Creative BioMart).. Culture supernatant was collected and total RNA was isolated at 72 h post-stimulation.Culture supernatant was collected and total RNA was isolated at 72 h post-stimulation.

Influence of Secretoglobin family 1A member 1 ( Scgb1a1 ) deficiency on alveolar macrophage (AM) phenotypes. (A) Flow cytometric analysis of mouse AMs (CD45 + , CD11c hi , and Siglec-F hi cells) from bronchoalveolar lavage fluid. (B) AMs from WT ( Scgb1a1 +/+ ) and KO ( Scgb1a1 –/ – ) mice were analyzed for phenotypic differences at 4, 8, 12, and 40 weeks of age.

Gene expression in AMs from Scgb1a1 KO mice across age groups. (A) Up-regulated and down-regulated genes in AMs at 4, 8, 12, and 40 weeks; (B–D) Volcano plots of significantly up-regulated (red) and down-regulated (blue) genes (FDR < 0.05); (E) Venn diagrams of the significantly changed genes. Venn diagrams of the significantly changed gene sets in AMs from KO mice at different ages including GO-Molecular function pathways (F) , GO-Biological process pathways (G) , KEGG-metabolism pathways (H) , and KEGG-Disease pathways (I) . FDR < 0.05 was considered significant; FC, fold change.

Comparison of the AM gene expression patterns in Scgb1a1 KO and WT mice. (A) Up-regulated and down-regulated genes in AMs at 4, 8, 12, and 40 weeks; (B–E) . Volcano plots of significantly up-regulated (red) and down-regulated (blue) genes (FDR < 0.05); (F) PCA plot of the variant feature of gene expression; (G) Venn diagrams of the significantly changed genes. Venn diagrams of the significantly changed gene sets in AMs from KO vs WT mice at different ages including GO-Molecular function pathways (H) , GO-Biological process pathways (I) , KEGG-metabolism pathways (J) , and KEGG-Disease pathways (K) . FDR < 0.05 was considered significant; FC, fold change.

Reviews

02/07/2022

Precise antibody validation, enhances our immunoprecipitation accuracy.

06/07/2019

Efficient protein purification, delivers high yield and purity consistently.

03/25/2019

Timely protein expression optimization, greatly boosts our research efforts.

Q&As

How does SCGB1A1 influence the response of lung tissue to environmental pollutants and allergens?

SCGB1A1 helps protect lung tissue from environmental pollutants and allergens, reducing the risk of damage and inflammation.

What are the mechanisms by which SCGB1A1 modulates immune cell recruitment and activation in the lungs?

SCGB1A1 influences immune cell behavior in the lungs, affecting responses to infections and inflammation.

How does SCGB1A1 (Secretoglobin Family 1A Member 1) function in respiratory tract defense and lung homeostasis?

SCGB1A1 is important for respiratory defense, contributing to the maintenance of lung health and protection against irritants.

What is the impact of SCGB1A1 in asthma and chronic obstructive pulmonary disease (COPD) pathogenesis?

SCGB1A1 is implicated in the pathogenesis of asthma and COPD, influencing airway inflammation and reactivity.

How do alterations in SCGB1A1 expression or function affect respiratory infections and healing after lung injury?

Changes in SCGB1A1 expression can impact the body's ability to fight respiratory infections and heal after lung injuries.

What role does SCGB1A1 play in modulating inflammatory responses in chronic lung diseases?

SCGB1A1 modulates inflammation in chronic lung conditions, potentially impacting disease progression.

How does SCGB1A1 contribute to mucus production and airway protection?

SCGB1A1 plays a role in mucus production, essential for trapping and clearing pathogens and particles from the airways.