Recombinant Human SCGB1A1 cell lysate

• Cat.No.: SCGB1A1-1891HCL
• Product Overview: Human SCGB1A1 / Uteroglobin derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: SCGB1A1 secretoglobin, family 1A, member 1 (uteroglobin) [ Homo sapiens ]
• Official Symbol: SCGB1A1
• Synonyms: SCGB1A1; secretoglobin, family 1A, member 1 (uteroglobin); UGB, uteroglobin; uteroglobin; CC10; CC16; CCSP; Uteroglobin (Clara cell specific 10 kD protein); blastokinin; urine protein 1; urinary protein 1; clara cells 10 kDa secretory protein; clara cell phospholipid-binding protein; UGB; UP1; UP-1; CCPBP
• Gene ID: 7356
• mRNA Refseq: NM_003357
• Protein Refseq: NP_003348
• MIM: 192020
• UniProt ID: P11684
• Chromosome Location: 11q12.3
• Pathway: FOXA1 transcription factor network, organism-specific biosystem; Prostaglandin Synthesis and Regulation, organism-specific biosystem
• Function: binding; phospholipase A2 inhibitor activity

Reviews

02/07/2022

Precise antibody validation, enhances our immunoprecipitation accuracy.

06/07/2019

Efficient protein purification, delivers high yield and purity consistently.

03/25/2019

Timely protein expression optimization, greatly boosts our research efforts.

Q&As

How does SCGB1A1 influence the response of lung tissue to environmental pollutants and allergens?

SCGB1A1 helps protect lung tissue from environmental pollutants and allergens, reducing the risk of damage and inflammation.

What are the mechanisms by which SCGB1A1 modulates immune cell recruitment and activation in the lungs?

SCGB1A1 influences immune cell behavior in the lungs, affecting responses to infections and inflammation.

How does SCGB1A1 (Secretoglobin Family 1A Member 1) function in respiratory tract defense and lung homeostasis?

SCGB1A1 is important for respiratory defense, contributing to the maintenance of lung health and protection against irritants.

What is the impact of SCGB1A1 in asthma and chronic obstructive pulmonary disease (COPD) pathogenesis?

SCGB1A1 is implicated in the pathogenesis of asthma and COPD, influencing airway inflammation and reactivity.

How do alterations in SCGB1A1 expression or function affect respiratory infections and healing after lung injury?

Changes in SCGB1A1 expression can impact the body's ability to fight respiratory infections and heal after lung injuries.

What role does SCGB1A1 play in modulating inflammatory responses in chronic lung diseases?

SCGB1A1 modulates inflammation in chronic lung conditions, potentially impacting disease progression.

How does SCGB1A1 contribute to mucus production and airway protection?

SCGB1A1 plays a role in mucus production, essential for trapping and clearing pathogens and particles from the airways.