Recombinant Rat Kit Ligand

• Cat.No.: Kitlg-62R
• Product Overview: Recombinant Rat Stem cell factor produced inE.Coliis a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is purified by proprietary chromatographic techniques.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117 (c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Glu-Ile-Cys.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM Tris pH-7.5.
• Solubility: It is recommended to reconstitute the lyophilizedStem cell factor in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀is determined by the dose-dependant stimulation of the proliferation of human MO7e cells which is < 10 ng/ml, corresponding to a specific activity of > 1 x 105 units/mg.
• Storage: Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution SCF should be stored at 4℃between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Kitlg KIT ligand [ Rattus norvegicus ]
• Synonyms: Kitlg; KIT ligand; Mgf; SCF; Kitl; Kitlg; FPH2; KL-1; SHEP7; DKFZp686F2250; KITLG; C-kit ligand; Stem cell factor; Mast cell growth factor;steel factor
• Gene ID: 60427
• mRNA Refseq: NM_021843
• Protein Refseq: NP_068615
• UniProt ID: P21581
• Chromosome Location: 7q13
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Melanogenesis; Pathways in cancer
• Function: cytokine activity; identical protein binding; protein binding; stem cell factor receptor binding