Recombinant Rat Gapdh Protein, His-tagged

• Cat.No.: Gapdh-1562R
• Product Overview: Recombinant Rat Gapdh Protein (Gly56-Phe284) with N-His tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Gly56-Phe284
• Description: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.5 kDa
  Accurate Molecular Mass: 26 kDa
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gapdh glyceraldehyde-3-phosphate dehydrogenase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gapdh
• Synonyms: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; BARS-38; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate; Gapd
• Gene ID: 24383
• mRNA Refseq: NM_017008
• Protein Refseq: NP_058704
• UniProt ID: P04797