Recombinant Human GAPDH Protein(Met 1-Glu 335), His-tagged

• Cat.No.: GAPDH-014H
• Product Overview: Recombinant Human GAPDH Protein(P04406) (Met 1-Glu 335) was expressed in E. coli, with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Protein length: Met 1-Glu 335
• Form: Supplied as sterile 50mM Tris, 30% glycerol, pH 7.5.
• Bio-activity: Measured by its ability to catalyze D-glyceraldehyde 3-phosphate to 3-phosphoglycerate, The specific activity is >2500pmols/min/ug.
• Molecular Mass: The recombinant human GAPDH comprises 346 amino acids and migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions as predicted.
• Purity: > 93 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens ]
• Official Symbol: GAPDH
• Synonyms: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; GAPD; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; G3PD; MGC88685
• Gene ID: 2597
• mRNA Refseq: NM_001256799
• Protein Refseq: NP_001243728
• MIM: 138400
• UniProt ID: P04406