Active Recombinant Human F2 Protein, His-tagged

• Cat.No.: F2-1103H
• Product Overview: Recombinant Human F2 (Accession # P00734) Met1-Glu622, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Thr328, Ile364 & Val515
• Form: Lyophilized from a 0.2 µm filtered solution in HEPES and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC.The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human F2, His-tagged has a calculated MW of 4 kDa (light chain A), 31 kDa (heavy chain B), 14 kDa (C-terminal fragment). In SDS-PAGE migrates as 12 kDa, 21 kDa and 23 kDa, reducing conditions.
• Protein length: Met1-Glu622
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: F2 coagulation factor II (thrombin) [ Homo sapiens ]
• Official Symbol: F2
• Synonyms: F2; coagulation factor II (thrombin); prothrombin; serine protease; prothrombin B-chain; PT; THPH1
• Gene ID: 2147
• mRNA Refseq: NM_000506
• Protein Refseq: NP_000497
• MIM: 176930
• UniProt ID: P00734
• Chromosome Location: 11p11-q12