Recombinant Ovine Prolactin Antagonist, 199aa

• Cat.No.: PRL-590
• Product Overview: Recombinant Ovine Prolactin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa. The Prolactin n is purified by proprietary chromatographic techniques.

Specification

• Species: Ovine
• Source: E.coli
• Tag: Non
• Description: Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3.
• Solubility: It is recommended to reconstitute the lyophilized Prolactin in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Amino Acid Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.
• Biological Activity:
  Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.
• Storage: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PRL prolactin [ Ovis aries ]
• Synonyms: Prolactin; PRL;prolactin
• Gene ID: 443317
• mRNA Refseq: NM_001009306
• Protein Refseq: NP_001009306
• UniProt ID: P01240