Recombinant Human PRL protein, His-tagged

• Cat.No.: PRL-459H
• Product Overview: Recombinant Human PRL (P01236) aa. (Leu29~Cys227) fused with His tag was produced in HEK293F cells.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu29~Cys227
• Description: This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.4kDa; Accurate Molecular Mass: 24&28kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 6.2.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: PRL prolactin [ Homo sapiens (human) ]
• Official Symbol: PRL
• Synonyms: PRL; prolactin; GHA1; decidual prolactin; growth hormone A1
• Gene ID: 5617
• mRNA Refseq: NM_000948.5
• Protein Refseq: NP_000939.1
• UniProt ID: P01236