Recombinant Mouse Wnt3a Protein, His-tagged

• Cat.No.: Wnt3a-585M
• Product Overview: Recombinant Mouse Wnt3a Protein (Met1-Phe290+DSSTTTTTTESGC) with N-His tag was expressed in E. coli.

Specification

• Description: Enables protein domain specific binding activity; signaling receptor binding activity; and transcription coactivator activity. Involved in several processes, including COP9 signalosome assembly; calcium ion transmembrane transport via low voltage-gated calcium channel; and nervous system development. Acts upstream of or within several processes, including animal organ development; chordate embryonic development; and positive regulation of macromolecule metabolic process. Located in cell surface and extracellular space. Is active in glutamatergic synapse. Is expressed in several structures, including alimentary system; central nervous system; embryo ectoderm; genitourinary system; and sensory organ. Orthologous to human WNT3A (Wnt family member 3A).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.1 kDa
  Accurate Molecular Mass: 41 kDa
• Protein length: Met1-Phe290+DSSTTTTTTESGC
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Wnt3a wingless-related MMTV integration site 3A [ Mus musculus (house mouse) ]
• Official Symbol: Wnt3a
• Synonyms: WNT3A; wingless-related MMTV integration site 3A; protein Wnt-3a; vestigial tail; vt; Wnt-3a
• Gene ID: 22416
• mRNA Refseq: NM_009522
• Protein Refseq: NP_033548
• UniProt ID: P27467