Recombinant Mouse Wee2 Protein, Myc/DDK-tagged

• Cat.No.: Wee2-6993M
• Product Overview: Purified recombinant protein of Mouse WEE1 homolog 2 (S. pombe) (Wee2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
• Molecular Mass: 62.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Wee2 WEE1 homolog 2 (S. pombe) [ Mus musculus (house mouse) ]
• Official Symbol: Wee2
• Synonyms: Wee2; WEE1 homolog 2 (S. pombe); Wee1B; Gm1065; wee1-like protein kinase 2; mWee1B; wee1-like protein kinase 1B; wee1B kinase; EC 2.7.10.2
• Gene ID: 381759
• mRNA Refseq: NM_201370
• Protein Refseq: NP_958758
• UniProt ID: Q66JT0