Recombinant Active Human WEE2 Protein (Full length), N-GST-tagged

• Cat.No.: WEE2-41H
• Product Overview: Recombinant full-length human WEE2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full Length
• Description: WEE2, also known as WEE1B, is a dual-specificity kinases that uniquely function to phosphorylate specific Tyr/Thr residues on CDKs. It belongs to WEE1 family that also includes WEE1 homolog 1 (WEE1, also designated as somatic WEE1A) and PKMYT1 (previously known as MYT1). It is conserved from yeast to humans and has been shown to suppress CDK1 activity by phosphorylating its inhibitory sites. A key M phase-promoting factor (MPF) inhibitory kinase in oocytes and is required for maintaining meiotic arrest.
• Bio-activity: The specific activity of WEE2 was determined to be 5.5 nmol/min/mg as per actity assay protocol.
• Purity: The purity of WEE2 was determined to be > 90 % by densitometry.
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: WEE2 WEE1 homolog 2 (S. pombe) [ Homo sapiens (human) ]
• Official Symbol: WEE2
• Synonyms: WEE2; WEE2 oocyte meiosis inhibiting kinase; OOMD5; WEE1B; wee1-like protein kinase 2; WEE1 homolog 2; wee1-like protein kinase 1B; wee1B kinase; EC 2.7.10.2
• Gene ID: 494551
• mRNA Refseq: NM_001105558
• Protein Refseq: NP_001099028
• MIM: 614084
• UniProt ID: P0C1S8