Recombinant Human WEE2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: WEE2-2594H
• Product Overview: WEE2 MS Standard C13 and N15-labeled recombinant protein (NP_001099028) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 62.7 kDa
• AA Sequence: MDDKDIDKELRQKLNFSYCEETEIEGQKKVEESREASSQTPEKGEVQDSEAKGTPPWTPLSNVHELDTSSEKDKESPDQILRTPVSHPLKCPETPAQPDSRSKLLPSDSPSTPKTMLSRLVISPTGKLPSRGPKHLKLTPAPLKDEMTSLALVNINPFTPESYKKLFLQSGGKRKIRGDLEEAGPEEGKGGLPAKRCVLRETNMASRYEKEFLEVEKIGVGEFGTVYKCIKRLDGCVYAIKRSMKTFTELSNENSALHEVYAHAVLGHHPHVVRYYSSWAEDDHMIIQNEYCNGGSLQAAISENTKSGNHFEEPKLKDILLQISLGLNYIHNSSMVHLDIKPSNIFICHKMQSESSGVIEEVENEADWFLSANVMYKIGDLGHATSINKPKVEEGDSRFLANEILQEDYRHLPKADIFALGLTIAVAAGAESLPTNGAAWHHIRKGNFPDVPQELSESFSSLLKNMIQPDAEQRPSAAALARNTVLRPSLGKTEELQQQLNLEKFKTATLERELREAQQAQSPQGYTHHGDTGVSGTHTGSRSTKRLVGGKSARSSSFTSGEREPLHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: WEE2 WEE2 oocyte meiosis inhibiting kinase [ Homo sapiens (human) ]
• Official Symbol: WEE2
• Synonyms: WEE2; WEE2 oocyte meiosis inhibiting kinase; OOMD5; WEE1B; wee1-like protein kinase 2; WEE1 homolog 2; wee1-like protein kinase 1B; wee1B kinase; EC 2.7.10.2
• Gene ID: 494551
• mRNA Refseq: NM_001105558
• Protein Refseq: NP_001099028
• MIM: 614084
• UniProt ID: P0C1S8