Recombinant Mouse Usp8 Protein, Myc/DDK-tagged

• Cat.No.: Usp8-6883M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin specific peptidase 8 (Usp8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 122.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Usp8 ubiquitin specific peptidase 8 [ Mus musculus (house mouse) ]
• Official Symbol: Usp8
• Synonyms: USP8; ubiquitin specific peptidase 8; ubiquitin carboxyl-terminal hydrolase 8; ubiquitin isopeptidase Y; ubiquitin thioesterase 8; deubiquitinating enzyme 8; ubiquitin thiolesterase 8; putative deubiquitinating enzyme; ubiquitin-specific-processing protease 8; Ubpy; AI574262; AW557536; mKIAA0055
• Gene ID: 84092
• mRNA Refseq: NM_019729
• Protein Refseq: NP_062703
• UniProt ID: Q80U87