Active Recombinant Human USP8, FLAG-tagged

• Cat.No.: USP8-86H
• Product Overview: Human USP8, amino-acids 2-1118(end) with an N-terminal FLAG-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Specification

• Description: Ubiquitin carboxyl-terminal hydrolase 8 is an enzyme that in humans is encoded by the USP8 gene.
• Source: Sf9 cell
• Species: Human
• Tag: FLAG
• Form: 45 mM Tris-HCl, pHUSP8 gel 8.0, 124 mM NaCl, 2.4 mM KCl, 90 μg/ml Flag peptide, 10% glycerol, and 3 mM DTT.
• Bio-activity: 246 pmol/min/μg Assay conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP8 in a 50 μL reaction. Reaction was incubated at 37°C for 15 min and fluorescent signal was measured at excitation= 340 nm, emission = 460 nm.
• Molecular Mass: 128 kDa
• Purity: >70%
• Applications: Useful for the study of enzymekinetics, screening inhibitors, and selectivityprofiling.
• Stability: >6 months at –80°C
• Protein length: 2-1118 a.a.

Gene Information

• Gene Name: USP8 ubiquitin specific peptidase 8 [ Homo sapiens ]
• Official Symbol: USP8
• Synonyms: USP8; ubiquitin specific peptidase 8; ubiquitin specific protease 8; ubiquitin carboxyl-terminal hydrolase 8; HumORF8; KIAA0055; UBPY; Deubiquitinating enzyme 8; FLJ34456; hUBPY; HumORF8; KIAA0055; MGC129718; Ubiquitin carboxyl terminal hydrolase 8; Ubiquitin carboxyl-terminal hydrolase 8; Ubiquitin isopeptidase Y; ubiquitin specific peptidase 8; Ubiquitin specific processing protease 8; Ubiquitin thioesterase 8; Ubiquitin thiolesterase 8; Ubiquitin-specific-processing protease 8; UBP8_HUMAN; UBPY; USP8; hUBPy; ubiquitin isopeptidase Y; ubiquitin thioesterase 8; deubiquitinating enzyme 8; ubiquitin thiolesterase 8; ubiquitin-specific-processing protease 8
• Gene ID: 9101
• mRNA Refseq: NM_001128610
• Protein Refseq: NP_001122082
• MIM: 603158
• UniProt ID: P40818
• Chromosome Location: 15q21.1
• Pathway: Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; ErbB2/ErbB3 signaling events, organism-specific biosystem; Internalization of ErbB1, organism-specific biosystem
• Function: SH3 domain binding; cysteine-type endopeptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity