Active Recombinant Human USP8

• Cat.No.: USP8-127H
• Product Overview: Recombinant Human USP8 was expressed in E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Ubiquitin carboxyl-terminal hydrolase 8 is an enzyme that in humans is encoded by the USP8 gene.
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of USP8 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of USP8 was compared confirming the deubiquitylating activity of USP8.
• Molecular Mass: ~128kDa
• Purity: >66% by InstantBlue™ SDS-PAGE
• Storage: 12 months at -70℃; aliquot as required.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: USP8 ubiquitin specific peptidase 8 [ Homo sapiens ]
• Official Symbol: USP8
• Synonyms: USP8; ubiquitin specific peptidase 8; ubiquitin specific protease 8; ubiquitin carboxyl-terminal hydrolase 8; HumORF8; KIAA0055; UBPY; hUBPy; ubiquitin isopeptidase Y; ubiquitin thioesterase 8; deubiquitinating enzyme 8; ubiquitin thiolesterase 8; ubiquitin-specific-processing protease 8; FLJ34456; MGC129718
• Gene ID: 9101
• mRNA Refseq: NM_001128610
• Protein Refseq: NP_001122082
• MIM: 603158
• UniProt ID: P40818
• Chromosome Location: 15q21.1
• Pathway: Downregulation of ERRB2:ERBB3 signaling, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; ErbB2/ErbB3 signaling events, organism-specific biosystem; Internalization of ErbB1, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by ERBB2, organism-specific biosystem
• Function: SH3 domain binding; cysteine-type endopeptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity