Recombinant Mouse Tyw5 Protein, Myc/DDK-tagged

• Cat.No.: Tyw5-6762M
• Product Overview: Purified recombinant protein of mouse full-length tRNA-yW synthesizing protein 5 (Tyw5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 36.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tyw5 tRNA-yW synthesizing protein 5 [ Mus musculus (house mouse) ]
• Official Symbol: Tyw5
• Synonyms: Tyw5; tRNA-yW synthesizing protein 5; 1110034B05Rik; tRNA wybutosine-synthesizing protein 5; jmjC domain-containing protein C2orf60 homolog; tRNA yW-synthesizing enzyme 5; tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase; EC 1.14.11.42
• Gene ID: 68736
• mRNA Refseq: NM_001037742
• Protein Refseq: NP_001032831
• UniProt ID: A2RSX7