Recombinant Mouse TNFSF12 protein(Arg105-His249), rFc-tagged

• Cat.No.: TNFSF12-810M
• Product Overview: Recombinant Mouse TNFSF12 (NP_035744.1) (Arg105-His249) was expressed in HEK293 with the Fc region of Rabbit IgG at the N-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N-rFc
• Protein length: Arg105-His249
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-50 ng/mL.
• Molecular Mass: The recombinant mouse TNFSF12 consists of 396 amino acids and predicts a molecular mass of 43.5 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 ％ as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfsf12 tumor necrosis factor (ligand) superfamily, member 12 [ Mus musculus ]
• Official Symbol: TNFSF12
• Synonyms: TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis; Dr3l; Apo3l; Dr3lg; Tweak
• Gene ID: 21944
• mRNA Refseq: NM_011614
• Protein Refseq: NP_035744