Recombinant Mouse Sost protein, His-tagged

• Cat.No.: Sost-753M
• Product Overview: Recombinant Mouse Sost protein(Q99P68)(Gln24-Tyr211), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N-His
• Protein length: Gln24-Tyr211
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse SOST, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 22 kDa. Due to glycosylation,the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Sost sclerostin [ Mus musculus ]
• Official Symbol: Sost
• Synonyms: SOST; sclerostin; 5430411E23Rik
• Gene ID: 74499
• mRNA Refseq: NM_024449
• Protein Refseq: NP_077769